Flow rate and solvent consumption in UHPLC decreased. Changes in mobile phase composition of the 2 methods were not required. Routine runtimes in HPLC and UHPLC took 40 and 15 minutes, respectively. The mean retention time, standard deviation, flow rate, and repeatability of analytical results were compared by using 2 methods. UHPLC analysis was performed using Knauer PAD detector with cooling autosampler and Eurospher II 100-3 C-18 column (100 mm × 3 mm) and also 2 pumps. HPLC analysis was performed using a Knauer by 100-5 C-18 column (250 mm × 4.6 mm) and Knauer photodiode array detector (PAD). In a case–control study, standard solutions of 7 benzodiazepines (BZs) and 73 biological samples such as urine, tissue, stomach content, and bile that screened positive for BZs were analyzed by HPLC and UHPLC in laboratory of forensic toxicology during 2012 to 2013. The aim of this study was to compare system efficiency and analysis duration regarding the solvent consumption and system maintenance in high-pressure liquid chromatography (HPLC) and ultra high-pressure liquid chromatography (UHPLC). Received in revised form February 9, 2015 This is an open access article distributed under the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The authors have no funding and conflicts of interest to disclose. From the Department of Forensic Medicine, School of Medicine, Tehran University of Medical Sciences (BB, AS) Tehran University of Medical Sciences, Faculty of Pharmacy, Toxicology and Poisoning Research Centre (AS) Laboratory of Forensic Toxicology, Legal Medicine Research Center of Tehran, Tehran, Iran (EB, AF) School of Nursing & Midwifery, Kermanshah University of Medical Sciences, Kermanshah, Iran (ES) Health Services Management, Kerman University of Medical Sciences, Kerman, Iran (SHSA).Ĭorrespondence: Ardeshir Sheikhazadi, Department of Forensic Medicine, School of Medicine, Tehran University of Medical Sciences, Poursina Street, Keshavarz Boulevard, Tehran 1417613151, Iran (e-mail: ).Ībbreviations: BZ = benzodiazepine, CVA = cerebrovascular accident, GC = gas chromatography, GC/MS = gas chromatography/mass spectrometry, HPLC = high-pressure liquid chromatography, IHD = ischemic heart diseases, LC = liquid chromatography, LMO = legal medicine organization, LOD = limit of detection, PAD = photodiode array detector, RT = retention time, TCA = tricyclic antidepressants, UHPLC = ultra high-pressure liquid chromatography, UPLC/MS = ultra pressure liquid chromatographymass spectrometry.Īrdeshir Sheikhazadi, Tehran University of Medical Sciences, Faculty of Pharmacy, Toxicology and Poisoning Research Centre Tehran, Iran (e-mail: ).
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